Formal:
* De quoi souhaitez-vous parler? (literally: "Of what do you wish to speak?") - This is the most formal option.
* Quel est le sujet de votre conversation? (literally: "What is the subject of your conversation?")
Informal:
* De quoi tu veux parler? (literally: "Of what do you want to speak?")
* Tu veux parler de quoi? (literally: "You want to speak of what?")
* On parle de quoi? (literally: "We speak of what?") - This is a bit more casual and can be used with friends.
You can also say:
* Qu'est-ce que tu voudrais discuter? (literally: "What would you like to discuss?")
* C'est quoi le sujet? (literally: "What is the subject?")
The best option will depend on your relationship with the person you're talking to and the context of the conversation.