Here are some ways to interpret this question:
* Literally: You could argue the period is the largest since it's a full stop.
* Historically: The full stop (period) is one of the oldest punctuation marks, dating back to ancient times.
* By visual size: The exclamation mark can be quite large and impactful in some fonts.
* By impact: A comma can drastically change the meaning of a sentence.
So, the answer really depends on how you define "largest"!