Here are a few contenders, depending on the criteria:
* By number of native English speakers: London is likely the second largest, after New York City.
* By total number of English speakers: London still holds a strong position, as it attracts a massive number of immigrants and visitors.
* By population: London is the largest city in the European Union and likely the second largest English-speaking city by population, after New York City.
Ultimately, there's no definitive answer without specifying the exact criteria for "largest."