1. As a statement: "Yes, we can." (A comma after "Yes" is used to separate the introductory word from the rest of the sentence.)
2. As a question: "Yes we can?" (A question mark at the end indicates it's a question.)
3. As an exclamation: "Yes! We can!" (An exclamation point after "Yes" emphasizes excitement or enthusiasm.)
4. As a title or heading: "Yes We Can" (Capitalize the first letter of each word and use no punctuation.)
Without further context, the most likely punctuation is: "Yes, we can."