If you are referring to the act of wearing the glasses:
* She - "She put on her glasses."
* They - "They helped her put her glasses on."
If you are referring to the glasses themselves as an object:
* Them - "She took them off her face."
* It - "The case held it securely." (if you are referring to a single lens)
If you are referring to the glasses in a more abstract sense:
* Her vision - "Her glasses helped her vision."
* Her sight - "She needed her glasses to improve her sight."
Ultimately, the best pronoun to use will depend on the specific situation and what you are trying to communicate.