General negativity:
* Gloomy
* Dour
* Sullen
* Morose
* Depressed
* Dispirited
Lack of enthusiasm:
* Dismal
* Unhappy
* Sad
* Melancholy
* Downcast
Specifically related to the tone of voice or expression:
* Grumpy
* Sour
* Peevish
* Cross
The best antonym for "cheery" will depend on the context of the sentence.