General Negativity:
* Gloomy
* Depressed
* Downcast
* Sad
* Miserable
* Unhappy
* Dispirited
* Melancholy
* Sombre (more formal)
Anger/Frustration:
* Angry
* Frustrated
* Irritated
* Cross
* Grumpy
* Cranky
Neutral/Lack of Emotion:
* Apathetic
* Indifferent
* Unenthusiastic
* Dour
* Grim
Specific Situations:
* Nervous (in contrast to being cheerfully confident)
* Worried (in contrast to being cheerfully optimistic)
* Anxious (in contrast to being cheerfully carefree)
The best antonym for "cheerful" will depend on the specific context in which you're using it.